Microglia are the resident brain immune cells, initiating local immune responses. It is well known that microglia, upon activation, show a broad polarization spectrum of phenotypes, determining their function in the brain. Specifically, microglial phenotypes range from pro-inflammatory or disease-stated to anti-inflammatory or homeostatic. Previous research has shown that a high-fat diet activates pro-inflammatory microglia. Two major high-fat diet related compounds that contribute to microglial inflammation are advanced glycation endproducts (AGEs), which are sugar-derived post-translational modifications of long-lived proteins, and palmitic acid, a saturated fatty acid. However, studies have indicated that exercise inhibits the activation of pro-inflammatory microglia. This inhibition might be caused by an exercise-inducible metabolite called N-lactoyl-phenylalanine (Lac-Phe). Recent evidence suggests that Lac-Phe suppresses obesity by reducing food intake, body weight and body fat. Besides, Lac-Phe seems to improve glucose homeostasis. Therefore, in this study, we investigate the effect of Lac-Phe on pro-inflammatory microglia caused by a high-fat diet. To study this, BV-2 cell culture experiments will be conducted. Here, BV-2 cells will be activated by AGEs or palmitic acid, after which the cells will be treated with Lac-Phe. We expect that Lac-Phe treatment will inhibit the pro-inflammatory activation of BV-2 cells caused by high-fat diet related compounds like AGEs and palmitic acid. However, this study is still ongoing and the results will be shown in the future.